Effect of 1-methylcyclopropene on ethylene-induced abscission in citrus
Identifieur interne : 002E48 ( Main/Exploration ); précédent : 002E47; suivant : 002E49Effect of 1-methylcyclopropene on ethylene-induced abscission in citrus
Auteurs : GUANGYAN ZHONG [Israël] ; Moshe Huberman [Israël] ; XU QIAO FENG [Israël] ; Edward C. Sisler [États-Unis] ; Doron Holland [Israël] ; Raphael Goren [Israël]Source :
- Physiologia Plantarum : (København. 1948) [ 0031-9317 ] ; 2001.
Descripteurs français
- Pascal (Inist)
English descriptors
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Abstract
Pre-treatment of citrus leaves and leaf explants (Citrus sinensis [L.] Osbeck cv. Shamouti), with 1-methylcyclopropene (1-MCP), induced endogenous ethylene production when leaves were further incubated in air. The induction of ethylene production was 1-MCP concentration-dependent. Abscission was concomitantly delayed. In leaves pre-treated with 1-MCP followed by exposure to ethylene, abscission was significantly delayed in comparison with those without 1-MCP pre-treatment. When leaf explants were co-treated for 24 h with ethylene and 1-MCP, abscission was delayed quite efficiently. The Lineweaver-Burke plot yielded a half-maximal value of 0.234 μl l-1 for the effect of ethylene on abscission. 1-MCP-1 competed kinetically with ethylene with a Ki value of approximately 1.4-5.5 nl l-1 1-MCP. Under these experimental conditions there was some competition between 1-MCP and ethylene. However, ethylene was not able to completely counteract the inhibitory effect of 1-MCP. Pre-treatment with 1-MCP, followed by exogenous ethylene treatment, suppressed the induction of endo-β-glucanase (EG) activity at the laminar abscission zone. The ethylene-dependent accumulation of the hydrolyse gene was demonstrated by blocking the accumulation of CsCel al mRNA by 1-MCP. Six hours of exposure of leaves to 1-MCP at various times during a total of 24 h ethylene treatment efficiently reversed ethylene induction of CsCel al gene at mRNA level up to 18 h. The results demonstrate that the induction of abscission by ethylene is controlled at mRNA level at the abscission zone.
Affiliations:
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Sisler</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Biochemistry, North Carolina State University</s1><s2>Raleigh, NC 17695-7622</s2><s3>USA</s3><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Holland, Doron" sort="Holland, Doron" uniqKey="Holland D" first="Doron" last="Holland">Doron Holland</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Institute of Horticulture, Agricultural Research Organization, The Vulcani Center</s1><s2>50250 Bet Dagan</s2><s3>ISR</s3><sZ>5 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Goren, Raphael" sort="Goren, Raphael" uniqKey="Goren R" first="Raphael" last="Goren">Raphael Goren</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Kennedy-Leigh Centre for Horticultural Research, Faculty of Agriculture, Food, and Environmental Quality Sciences, The Hebrew University of Jerusalem</s1><s2>76100 Rehovot</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>76100 Rehovot</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Physiologia Plantarum : (København. 1948)</title><title level="j" type="abbreviated">Physiol. Plant. : (Kbh., 1948)</title><idno type="ISSN">0031-9317</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Physiologia Plantarum : (København. 1948)</title><title level="j" type="abbreviated">Physiol. Plant. : (Kbh., 1948)</title><idno type="ISSN">0031-9317</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abscission</term><term>Biosynthesis</term><term>Citrus sinensis</term><term>Dose activity relation</term><term>Enzymatic activity</term><term>Ethylene</term><term>Gene</term><term>Gene expression</term><term>Induction</term><term>Plant leaf</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Abscission</term><term>Feuille végétal</term><term>Biosynthèse</term><term>Induction</term><term>Relation dose réponse</term><term>Expression génique</term><term>Gène</term><term>Activité enzymatique</term><term>Citrus sinensis</term><term>Ethylène</term><term>Endo-β-glucanase</term><term>1-Méthylcyclopropène</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pre-treatment of citrus leaves and leaf explants (Citrus sinensis [L.] Osbeck cv. Shamouti), with 1-methylcyclopropene (1-MCP), induced endogenous ethylene production when leaves were further incubated in air. The induction of ethylene production was 1-MCP concentration-dependent. Abscission was concomitantly delayed. In leaves pre-treated with 1-MCP followed by exposure to ethylene, abscission was significantly delayed in comparison with those without 1-MCP pre-treatment. When leaf explants were co-treated for 24 h with ethylene and 1-MCP, abscission was delayed quite efficiently. The Lineweaver-Burke plot yielded a half-maximal value of 0.234 μl l<sup>-1</sup> for the effect of ethylene on abscission. 1-MCP<sup>-1</sup> competed kinetically with ethylene with a K<sub>i</sub> value of approximately 1.4-5.5 nl l<sup>-1</sup> 1-MCP. Under these experimental conditions there was some competition between 1-MCP and ethylene. However, ethylene was not able to completely counteract the inhibitory effect of 1-MCP. Pre-treatment with 1-MCP, followed by exogenous ethylene treatment, suppressed the induction of endo-β-glucanase (EG) activity at the laminar abscission zone. The ethylene-dependent accumulation of the hydrolyse gene was demonstrated by blocking the accumulation of CsCel al mRNA by 1-MCP. Six hours of exposure of leaves to 1-MCP at various times during a total of 24 h ethylene treatment efficiently reversed ethylene induction of CsCel al gene at mRNA level up to 18 h. The results demonstrate that the induction of abscission by ethylene is controlled at mRNA level at the abscission zone.</div></front></TEI><affiliations><list><country><li>Israël</li><li>États-Unis</li></country><region><li>Caroline du Nord</li></region></list><tree><country name="Israël"><noRegion><name sortKey="Guangyan Zhong" sort="Guangyan Zhong" uniqKey="Guangyan Zhong" last="Guangyan Zhong">GUANGYAN ZHONG</name></noRegion><name sortKey="Goren, Raphael" sort="Goren, Raphael" uniqKey="Goren R" first="Raphael" last="Goren">Raphael Goren</name><name sortKey="Holland, Doron" sort="Holland, Doron" uniqKey="Holland D" first="Doron" last="Holland">Doron Holland</name><name sortKey="Huberman, Moshe" sort="Huberman, Moshe" uniqKey="Huberman M" first="Moshe" last="Huberman">Moshe Huberman</name><name sortKey="Xu Qiao Feng" sort="Xu Qiao Feng" uniqKey="Xu Qiao Feng" last="Xu Qiao Feng">XU QIAO FENG</name></country><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Sisler, Edward C" sort="Sisler, Edward C" uniqKey="Sisler E" first="Edward C." last="Sisler">Edward C. Sisler</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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